SOY BEAN ISOFLAVONE

Antiestrogenic, Antifungal, Antiviral and Antioxidant Activities






Soybean is not widely consumed as food source in the West, but it is very important food in Japan and Asian countries. Isoflavones are found in soybeans in large amount and contain phytochemical compounds with antiestrogenic, antifungal, antiviral and antioxidant activities.

Isoflavone-intake serves to protect osteoporosis since soybean isoflavones suppress bone resorption.

Etiologic data show that higher frequency of prostatic carcinoma was found in white and black people than Japanese. The Finnish people's blood contains less isoflavone than Japanese. The isoflavone contents in blood is related to carcinogenesis. It was found that it keeps a mouse liver from nitrosoamine-induced cancer to feed a soybean diet. Also, biochemical data supported these observations. One of isoflavones, Genistein, shows to inhibit the growth of human breast cancer cell, tyrosine protein kinases and to block angiogenesis. Thus, isoflavone has potentiality for protection of carcinogenesis.






SPECIFICATIONS for Soy Bean ISOFLAVONE-10

* Soy Bean ISOFLAVONE-10 contains not less than 10.0% of isoflavone glycosides on dry matter.

Description:........Pale yellow powder
Purity Test
(1) Heavy metals ........ less than 20 ppm
(2) Arsenic ............. less than 2 ppm
Loss on drying .......... less than 6.0% (per gram, 105 deg.C., 1 hr)
Ash ..................... less than 5.0% (per gram)

CONTENTS OF ISOFLAVONE GLYCOSIDES
TOTAL ................... not less than 10.0%
DAIDZEIN GLYCOSIDES ..... 4.0 to 6.0%
GLYCITEIN GLYCOSIDES .... 2.0 to 4.0%
GENISTEIN GLYCOSIDES .... 1.0 to 3.0%


ANALYSIS CERTIFICATE (for Lot #78130)
Description .............. Pale yellow powder
Purity Test
(1)Heavy Metals ....... Less than 20 ppm
(2) Arsenic ........... Less than 2 ppm
Loss on Drying ....... 3.1%
Ash .................. 1.3%

Content of Isoflavone Glycosides ........ 10.1%
(1) Daidzein Glycosides ...... 5.2%
(2) Glycitein Glycosides ..... 3.0%
(3) Genistein Glycosides ..... 1.9%
Microbial Limits
(1) Total aerobic plate count ...... Less than 1000 count per gram
(2) E. Coli ........................ negative


Note:

Weight accurately approx. 100mg and add in 20ml of methanol/sulfuric acid/water (8:1:1). Dissolve, dilute with methanol to exactly 50ml. Stopper tightly and heat for one hour at 100 deg. C. Cool and filter this solution(=the Sample Solution).

Weigh accurately approx. each 2mg of Daidzein and Genistein (supplied by SIGMA CHEMICAL COMPANY), dissolve in 50ml of methanol(=the Standard solution).

Inject 10 micro liter of each solution to High Performance Liquid Chromatograph (HPLC) respectively. Measure the peak areas of Daidzein, Glycitein and Genistein on the chromatograms of the sample solution and Daidzein and Genistein on the chromatograms of the Standard solution.

Operating conditions of HPLC
Column..... Shiseido Capcell Pak C18 (4.6 x 150mm)
Mobile phase ... 0.1% Trifluoroacetic acid solution/Acetonitrile = 75/25
Flow rate....... 1.0ml/min.
Detector ....... UV Absorption 260nm


DOSAGE:
Recommended daily dosage is 150mg to 200mg as material.



ISOFLAVONE HIGHLY CONTAINING MATERIALS:
SPECIFICATIONS for Soy Bean ISOFLAVONE-20, -40 & -80
Descriptions Yellowish brown powder with bitter taste and characteristic odor
ISOFLAVONE Not less than 20% (ISOFLAVONE-20)
Not less than 40% (ISOFLAVONE-40)
Not less than 80% (ISOFLAVONE-80)
Heavy metals Not more than 10 ppm
Arsenic Not more than 1 ppm
Loss on drying Not more than 5% (ISOFLAVONE-20 & 40)
Not more than 6% (ISOFLAVONE-80)
General bacteria Not more than 300/g
Coliform bacilli Negative









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