PERILLA SEED EXTRACT POWDER & LIQUID

Effective anti-allergy substance

** Perilla Leaf Extract Powder is also available.



Product Standards for Perilla Seed Extract
Flavonoids in polyphenol are extracted from perilla (perilla frutescens var. japonica or crispa) seeds in ethanol.
Two forms of Perilla Seed Extract are availablel - Perilla Seed Extract Powder and Perilla Seed Extract Liquid.




SPECIFICATIONS for Perilla Seed Extract - P (Powder)
Appearance yellowish powder with bitter taste
Identification yellow
(DAVIS method)
Content of Polyphenol more than 3%
(Folin-Denis method)
Loss on drying less than 5%
(Analysis for Hygienic Chemists,1g, 105, 2h)
Standard plate counts less than 3,000 cells/g
(Analysis for Hygienic Chemists)
Coliforms Negative
(Analysis for Hygienic Chemists)
Arsenic less than 1 ppm
(Standard Methods of Analysis in Food Safety Regulation)
Heavy metals less than 30 ppm
(The Japanese Standards for Food Additives)
Moulds and Yeasts less than 1,000 cells/g
(Analysis for Hygienic Chemists)
**Certification Test - To 1.0g of this product, add 8.5ml of 90% diethlene glycol and 0.5ml of 1N NaOH. Then a yellow color develops. (DAVIS method)

Certificate of Analysis dated March 7, 2003 for Perilla Seed Extract - P (Powder) Lot No. Y-225
Date of Product: November 25, 2002
Expiration Date: November 24, 2004
Appearance..... yellowish powder with bitter taste
Identification..... yellow (DAVIS method)
Content of Polyphenol..... 3.4% (Folin-Denis method)
Loss on drying..... 4.4% (Analysis for Hygienic Chemists,1g, 105, 2h)
Standard plate counts..... less than 1,000 cells/g
Coliforms..... Negative
Arsenic..... less than 1 ppm
Heavy metals..... less than 10 ppm
Moulds and Yeasts..... less than 100 cells/g


SPECIFICATIONS for Perilla Seed Extract - L (Liquid)
Appearance..... dark brown liquid with bitter taste
Content of Polyphenol..... more than 2% (Folin-Denis method)
Residue on Evaporation..... in the range of 18 to 22% (Analysis for Hygienic Chemists)
Standard plate counts..... less than 3,000 cells/g (Analysis for Hygienic Chemists)
Coliforms..... Negative (Analysis for Hygienic Chemists)
Arsenic..... less than 1 ppm (Standard Methods of Analysis in Food Safety Regulation)
Heavy metals..... less than 20 ppm (The Japanese Standards for Food Additives)
Moulds and Yeasts..... less than 1,000 cells/g (Analysis for Hygienic Chemists)

**Certification Test - To 1.0g of this product, add 8.5ml of 90% diethlene glycol and 0.5ml of 1N NaOH. Then a yellow color develops. (DAVIS method)


Residual Agricultural Chemicals
BHC not detected (D/L=0.02ppm)
DDT not detected (D/L=0.02ppm)
Aldrin not detected (D/L=0.01ppm)
Dieldrin not detected (D/L=0.01ppm)
Endrin not detected (D/L=0.01ppm)
Diazinon not detected (D/L=0.05ppm)
Parathion not detected (D/L=0.05ppm)
Malathon not detected (D/L=0.05ppm)

D/L= Detection Limit
Test Method: Bas Chromatography
Tested by: Japan Food Research Center Foundation
Research results issue number 397060549-002
Acute Toxicity and Safety
LD50(mouse) More than 2000mg/kg
After administering 7.0g/day for two consecutive weeks on human, no toxic effects were observed.

Daily Dosage of PERILLA SEED EXTRACT
PERILLA SEED EXTRACT-P (powder) 100 - 150mg/day
PERILLA SEED EXTRACT-L (liquid) 150 - 225mg/day


What is Perilla Seed and Perilla Seed Extract?
Perilla frutescens and a lot of herbs like lavandula belong to Fam LABIATAE.
Perilla seed has been cultivated in Japan, China, Korea and Southeast Asia and used as spices and also as a major supply for -linolenate rich oil.
People with allergies are increasing in number every year, and the diet is said to be one of the most important factor for this treatment.
A daily intake of n-6 fatty acids contained in meat or plant oil such as safflower, is accumulated in the body as arachidonic acid which is converted to allergic mediators.
PERILLA SEED EXTRACT inhibits the production of allergic mediators, termed as leukotrienes (LTs).
The mediators are metabolites of arachidonic acid through 5-lipoxygenase (5-LO) pathway.
PERILLA SEED EXTRACT selectively inhibits 5-LO activity (in vitro).
PERILLA SEED EXTRACT also inhibits type IV allergy as well as TPA-induced inflammation (in vivo).
Moreover, the serum IgE levels are suppressed by administration of PERILLA SEED EXTRACT, and in line with this, allergic rhinitis, hay fever and urticaria are significantly improved.




Product Standards for Perilla "Leaf" Extract
The material used is limited to Perilla leaf from Hokkaido (Japan) from the view point of its quality, its anti-allergic effects and the control of its production. The variety used in the manufacturing process is Perilla frutescens var. acuta Kudo forma viridis Makino.


SPECIFICATIONS for Perilla Leaf Extract Powder
Characteristic Milky-white powder, slightly characteristic
odor and taste
pH (10% aqueous solution) 4.50 - 6.50
Brix (10% aqueous solution) 9.0 - 11.0
Moisture Not more than 5%
Heavy Metals (as Pb) Not detected (not more than 10ppm)
Arsenic (As As2O3) Not detected (not more than 0.2ppm)
Number of Bacteria Not more than 1000cfu/g
Mold and Yeast Not detected
Coliforms Negative
Expiration date: 2 years after the date of manufacture

EXTRACTION OF PERILLA LEAF
The decoction of the dried leaves afforded by boiling water gives an extract with greater anti-allergy activity than that obtained using aqueous ethanol and avoids the precipitation problems caused by chlorophyll etc., in the aqueous ethanol extract (Kosuna et al., 1994).


SPRAY DRYING
By the addition of dextrin to the aqueous Perilla extract, followed by spray drying, a stable powdered form is achieved, namely, Perilla extract powder with 1g equivalent to 1ml of Perilla extract. This powder is available for capsule and tablet formulations and for use in a range of health products.


MANUFACTURING PROCESS
Dried Perilla Leaves .....Extracted with boiling water .....Filtrate.....Purified.....Sterilized .....Added ethanl (for preservation) .....Product ..... Filling



Exclusively exported by YBS Corporation




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